ABSTRACT
Silver nanoparticles have gained significant attention due to their unique properties and versatile applications in various fields including medicine, electronics, and catalysis. This study focuses on the use of the cell-free enzyme of Pseudomonas aeruginosa for the synthesis of silver nanoparticles by biological method and its application as an antimicrobial agent. Standard biological method was followed for the synthesis of silver nanoparticles using cell-free enzyme of Pseudomonas aeruginosa. Characterization of silver nanoparticles was carried out using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Electron Dispersive X-ray Diffraction (EDX). The antimicrobial potential of synthesized nanoparticles was tested against clinical isolates using standard microbiological methods. However, on UV-vis spectroscopy, the adsorption peak was formed at 400 nm. The XRD spectrum exhibited 2Ø values of 23.00⁰(111), 25.24⁰(200) ,29.95⁰(231), 34.00⁰(142), 47.98⁰(241), 56.00⁰(220), 59.97⁰(311), 63.98⁰(222) corresponding to bragg’s reflection. There was an increase in transmittance on FTIR peaks formed at 2948cm-1 and a decrease at 3396m-1. SEM images produced particles that adhered to each other with large agglomerations and this confirmed that the sample consists of silver nanoparticles. The EDX showed that the synthesized nanoparticles consist of silver, carbon, oxygen, and trace amounts of phosphorus, chloride, and calcium. Antimicrobial profile of both silver nanoparticles (1000mg/L) and silver metal has stronger inhibition, particularly against Escherichia coli (ESBL) with a 17mm zone of inhibition. Silver nanoparticles were effective against Escherichia coli and Pseudomonas sp. at 12mm zone of inhibition. However, some strains like Bacillus subtilis and Staphylococcus aureus (MRSA) displayed lower susceptibility. This study also analyzes the effectiveness of silver nanoparticles as antimicrobial agents, with a promising investigation for clinical applications.
