SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES BY CELLFREE ENZYME OF Carnobacterium gallinarum AND ITS APPLICATION AS AN ANTIMICROBIAL

ABSTRACT

Nanoparticles are now used in a wide range of industrial, biomedical and environmental applications. This study was carried out to synthesise and characterise silver nanoparticles using cell-free enzymes of Carnobacterium gallinarum and assess its applications as antibiotic agents. 14 Cell-free enzymes were extracted from Carnobacterium gallinarum using standard analytical procedures and the synthesised nanoparticles were characterised using UV-vis spectroscopy, FTIR, X-ray Diffraction (XRD), Scanning Emission Microscopy (SEM) and Energy Dispersive X-ray (EDX). The results of the UV-vis showed that Ag nanoparticles were formed at 400nm with strong FTIR peaks at 2937cm-1 and 3375cm-1 carbon stretch. XRD showed the presence of silver in nine planes with percentage concentration by weight ranging from 1.76 to 32.20%. The shape of the nanoparticles was spherical with a particle size of 31.14 nm, height of 1.76 mm and bulk density of 0.40 g/cm3 . The percentage composition of the metals in the nanoparticles was as follows: Ag (70.43%), carbon (14.72%), oxygen (6.98%), while P, Cl, Ca occurred in trace amounts. Ag nanoparticles demonstrated antimicrobial activity against some clinical isolates but were most effective against Pseudomonas sp. (MDR). It was then concluded that cell-free enzymes of Carnobacterium gallinarum can be used in the synthesis of Ag nanoparticles and these nanoparticles have antimicrobial ability.