ABSTRACT
The study was carried out to identify the microorganism and phytochemicals present in Alstonia boonei. The experiment was conducted between 8th of December, 2021 to 8th of February, 2022 at the Department of Crop Science’s Experimental Farm open field, Faculty of Agriculture, University of Benin, Benin City. The plant parts of Alstonia boonei were collected from Forestry Nursery, Faculty of Agriculture, and University of Benin, Benin city. The Roots, stem and leaves of Alstonia boonei were thoroughly washed, dried, homogenized and stored at -200C prior to analysis. Phytochemical constituent such as Alkaloid, flavonoid, phenol, saponin and tannin were evaluated. Microbial analysis was also carried out to identification the fungal and bacteria isolates associated with Alstonia boonei. From the result of the study, it was observed that Fusarium, Trichoderma spp. were the dominant fungi species while proteus spp. Staphylococus and staphylococcus were the most dominant bacteria species. Results of the phytochemical screening showed the presence and quantity of Alkaloid, Flavonoid, phenol Saponin and Tanin in the Root, Stem and leave of Alstonia boonei. The quantity of tannin was highest in the Root and lowest in the leaf with a value. The presence of tannins in extract of leaf, stem and root of Alstonia boonei showed that the plants can be used as purgative. This study indicates that all part of the A. boonei. contains phytochemicals/ useful microorganism but the leaf of A. boonei is very rich in phytochemicals. They are known to show medicinal activity as well as exhibiting physiological activity. Hence, the society should be educated on xii the nutritional, medicinal and economic values of the leaves of A. boonei. As increase in the awareness of the usefulness of the plant part can result in an increase in the demand for the plant which in turn will go a long way of reducing the quantity of environmental waste from the plant part.
