EVALUATING THE HAEMATOLOGICAL AND BIOCHEMICAL EFFECT OF AMLODIPINE AND VARSATAN ON THE ANTIDIABETIC ACTIVITY OF PIOGLITAZONE IN STREPTOZOTOCIN INDUCED DIABETIC RAT

SUMMARY

To the   reagent blank test   tubes, 0.02 ml of   distilled water and   1 ml of   reagent 1(blank) were added. 0.02 ml of serum and 1 ml of reagent 1   were added to the sample   test tubes. 0.02 ml of standard and 1 ml reagent 1 were added to the standard test tubes.   The solutions in   the test tubes were   mixed and incubated   at 25˚C for   30 minutes. The absorbance   of the sample   and the standard against the   reagent blank was   measured at 546   nm (Tietz, 1995). Total protein was calculated as thus: Total protein (g/dl) = 19 x Absorbance of sample