ABSTRACT
Biofilms are biological systems where bacteria organize themselves into a coordinated functional community. They are formed as a response to a number of different factors. Haemolysins on the other hand, are lipids and proteins that lyse red blood cells by disrupting the cell mrmbrane. This research was carried out to evaluate the virulence factors and susceptibility pattern of some bacterial isolates: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Psuedomonas aeruginosa. Laboratory analysis was carried out to determine the antibacterial susceptibility patterns, the hemolytic activity and the biofilm forming capacity of the clinical bacterial isolates. Staphylococcus aureus showed sensitivity to rocephin (20 µg), gentamycin (10 µg) and levofloxacin (20 µg). It however showed high resistance to norfloxacin (10 µg) ampicillin (20 µg) and septrin (20 µg). Staphylococcus epidermidis on the other had showed no resistance to any of the antibiotics and was sensitive to all of them. Escherichia colishowed full sensitivity to sparfloxacin (10 µg), ciprofloxacin (30 µg), amoxicillin (30 µg), pefloxacin (30 µg) and streptomycin (30 µg). Isolates of the bacteria studied showed resistance to tarivid (10 µg). Pseudomonas aeruginosa isolates in this study showed resistance to streptomycin (30 µg) and septrin (30 µg) while showing sensitivity to sparfloxacin (10 µg), ciprofloxacin (30 µg), pefloxacin (30 µg) and tarivid (10 µg). Analysis revealed that Staphylococcus aureus, Escherichia coli, and Psuedomonas aeruginosa isolates studied in this research work showed beta hemolysis with just Staphylococcus epidermidis showing gamma hemolysis. The results also showed that Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa formed strong biofilms while Escherichia coli formed weak biofilms. It is recommended that indiscriminate use of broad-spectrum antibiotics and non-adherence to hospital antibiotic policies should be strongly discouraged. Also, antibiotic susceptibility pattern of bacterial pathogens in specialized clinical should be continuously monitored.
beta hemolysis with just Staphylococcus epidermidis showing gamma hemolysis. The results also showed that Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa formed strong biofilms while Escherichia coli formed weak biofilms. It is recommended that indiscriminate use of broad-spectrum antibiotics and non-adherence to hospital antibiotic policies should be strongly discouraged. Also, antibiotic susceptibility pattern of bacterial pathogens in specialized clinical should be continuously monitored.
