ABSTRACT
The vagina is an elastic canal which connects the uterus to the outside of the body, allowing for sexual intercourse, menstruation and childbirth. The vagina is a complex organ, comprising of a broad range of unicellular organisms. In the right conditions and proportions, the bacteria found in the vagina are not pathogenic to the host but in unusual circumstances, some of these bacteria can become parasitic or pathogenic. Antibiotic sensitivity is the susceptibility of bacteria to antibiotics. This testing provides information on the bacteria and possible treatment plans. The susceptibility testing is carried out in the laboratory in conditions that mimic those of the target habitat. This testing is done not only to determine the antibiotic that will be most effective in dealing with an infection, but also the exact levels of effectiveness of the antibiotic to the bacteria. The vagina is self-lubricating and self-cleaning and these processes involve the expulsion of a fluid known as vaginal discharge. This secretion comes from glands inside the vagina and cervix and its quantity and physical properties vary from woman to woman and is dependent on various factors such as menstrual cycle, presence of an infection, arousal, arousal, allergy, or even stress. The aim of this study is to elucidate the frequency of various bacteria in high vaginal swabs (HVS) and the sensitivity pattern to antibiotics. Swabs were cultured and inoculated on blood MacConkey agar. After overnight incubation, plates were examined for growth and colonial morphology. Final confirmation was done by modified Kirby-Bauer's dose diffusion method using Ceftazidime (30mg), Cefuroxime (30mg), Gentamicin (10mg), Ceftriamine (30mg), Erythromycin (5mg), Cloxacillin (5mg), Ofloxacin (5mg), Augumentin (30mg), Cefixime (5mg), Nitrofurantoin (300mg) and Ciprofloxacin (5mg). After twenty four hours, incubation plates were examined to read the zone of inhibition. Results showed that the most sensitive antibiotics were Ciprofloxacin (5mg) and Gentamicin (10mg) to Gram negative and Gram positive bacteria respectively, whereas the least effective were Augumentin (30mg) and Erythromycin (5mg) to Gram negative and Gram positive bacteria respectively. In conclusion, high prevalence of gynaecological infections demands that the patients who have infections must be investigated regularly and carefully through culture and identification of causative bacteria. Emergence of antibiotic resistance must be controlled in order to avoid improper use, frequent abuse and insufficient dosages. Trouble-free availability of antibiotics and treatment schedules must be designed subsequent to proper laboratory functions.
