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ABSTRACT
Antimicrobial resistance poses a significant global health threat, necessitating continuous surveillance and understanding of bacterial susceptibility patterns to guide effective treatment strategies. This project focuses on generating antibiogram, a comprehensive tool for assessing the susceptibility and resistance profiles of clinical isolates to commercially available antibiotics. The study incorporates bacterial isolates, including Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus, which are representative of common pathogens encountered in clinical settings. Laboratory techniques such as antimicrobial susceptibility testing (AST), following standardized protocols, were employed to assess the response of bacterial isolates to a panel of antibiotics. Mueller-Hinton Agar served as the culture medium to facilitate consistent and standardized conditions for AST. Results reveal critical insights into the susceptibility patterns of these clinical isolates to a range of antibiotics, providing essential information for clinical decision-making. The antibiogram generated from this study aids healthcare professionals in selecting appropriate antibiotics, thereby enhancing patient care and contributing to the global efforts to combat antimicrobial resistance. By illuminating the dynamic relationship between bacteria and antibiotics, this project contributes to the growing body of knowledge required to address the evolving challenge of antimicrobial resistance. Additionally, it underscores the importance of adherence to standard laboratory practices and the role of microbiology in guiding evidence-based healthcare interventions. This project underscores the significance of continuous surveillance and monitoring of antimicrobial susceptibility patterns, serving as a valuable resource for clinicians, microbiologists, and healthcare policymakers in the ongoing battle against antibiotic-resistant pathogens.
