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ABSTRACT
Nanoparticles (NPs) are substances that range from 1 to 100 nm in size. They have been applied in various fields, including material science and biotechnology, in recent years. Thus, the synthesis of nanoparticles can be considered a dynamic area of research and application. This study was aimed at exploring different methods for phytosynthesizing zinc oxide nanoparticles (ZONPs) and evaluating the antibacterial activity of the phytosynthesized nanoparticles over 5 days. Phytopathogens were isolated from a diseased tomato plant and characterized using morphological and biochemical methods. Zinc oxide nanoparticles were synthesized using a zinc oxide precursor solution and moringa leaf extract. Spectrophotometric analysis was used to characterize the phytosynthesized ZONPs. The effects of various methods of synthesis, incubation period, and concentration of ZONPS on the antibacterial activity were tested. Pseudomonas flourence was the isolated pathogen. The results of the spectrophotometric analysis showed a peak between 330 and 350 nm, indicating ZONP synthesis. The best method of NP synthesis that resulted in the highest antibacterial activity against Pseudomonas fluorescence was stirring the precursor solution and zinc oxide nanoparticles with heat. An incubation period between 24 and 48 hours resulted in ZONPs that were most effective for antibacterial activity. The concentration of ZONPs did not impact their antibacterial effectiveness. The findings of this study reveal that ZONPs synthesized with stirring and heating and incubated for 24-48 hours were very effective against the test pathogen.
